培養した鶏胎膵組織の観察

特にラ氏島細胞の再生について

抄録

Phasecontrastmicroscopical and histochemical analysis were performed to the cultivated pancreas-tissues of chickembryos at their various periods of incubation.
Pancreas-tissues of chickembryo, when cultivated in vitro in roller tubes, were hept alive during about 5 days or longer in the adequate fluid medium, and luxarious epitheliar sheeths and mitosis of the epitheliar cells could be obtained frequently. A part of these epitheliar cells, belonged to the primary undifferentiated pancreatic cells, and acinar cells, which were differentiated from the former cells, were most constantly found and analized by the Azan reaction and Jacobson's stain. But, the regenerations of pancreatic islet cells were not found so frequently, and when we utilized the pancreas from the chickembryos of 7-9 day's incubation, we could occationally found the primary β-islet cells, that had gomoriphilic protophasmic granules to the Gomori's C.H.P. stain. The periphery of the outgrowths, in general, were more gomoriphilic than the central portion of the epitheliar sheeths, then, this portion was suggested to be functionally most important in the whole area of the outgrowths.