絨毛組織細胞内Gonadotrophinの生物学的性質に関する研究

抄録

The extracts of human chorionic tissues and hydatidiform moles were prepared by homogenization and high speed centrifugation. The particle free supernatant fraction precipitated by ethanol and purified samples of urinary HCG assaying 5,100 I.U. and 2,406 I.U. per mg. were subjected to zone electrophoresis in starch grain.
Proteins recovered after electrophoresis from portions of the starch grain were tested for gonadotropic activities by the weight of rat ovaries, seminal vesicles and the augmentation test of Steelman & Pohley. There were found at least two biologically active components (G-A and G-B) in all of the extracts and preparations examined. G-A has luteinizing activity and G-B has follicle stimulating activity. Each electrophoretically homogeneous component could be isolated by repeated electrophoresis.
The extracts of human chorionic tissues in the early stage of pregnancy have predominant G-B activity and in the late stage, G-A activity when assayed by the ovarian weight method. The extracts of hydatidiform moles have more predominant G-B activity than that of normal chorionic tissues when assayed by the augmentation test of Steelman & Pohley.
The urea denaturatuion experiments and RDE digestions show that there may be differences between urinary HCG and trophoblastic HCG, between G-A of urinary HCG and G-A of urinary HMG.
The acetone powder of the supernatant fraction of hydatidiform moles has the specific component with gonadotropic activity, assayed by the augmentation test of Steelman & Pohley, in the zone of segment number 35-39, which is not found in the case of normal chorionic tissues.