抄録
A method for the estimation of 17-hydroxycorticoids in urine is described. In its greater parts the Thorn's method is followed: the serial extractions with butanol at pH 1.0 and the colorimetric measurement of 17-hydroxycorticoids with phenylhydrazine sulfuric acid reaction.
For the elimination of the nonspecific chromogens in urine the semicarbazide is used instead of the alkaline wash. The addition of the semicarbazide can eliminate the greater parts of them than alkaline wash. Therefore the estimation of corticoids can be easily performed without the reference of the increases of the nonspecific chromogens, and it naturally makes it possible to store the urine specimens at room temperature.
Use of this method in clinical studies, being not so many, demonstrated good correlation with known states of adrenocortical function.
