By means of acetone precipitation, a substance was prepared from the fraction obtained by removing parotin from the autolyzed bovine parotid gland. Its biological properties were similar to parotin possessing the hypocalcemic and leucocytosis-producing activities for rabbits. This parotin-like substance was named β-parotin. Autolysis at 37°C for 24hrs, was most effective for preparing β-parotin. The parotin obtained from the autolyzed gland was also active in both effects.
