抄録
The whole testis tissue preparation of rat or man was incubated with androst-4-ene-3β, 17β-diol (4ADL)(20 or 40μg in one flask containning 1 or 2 testes) for 2 hrs.in Krebs-Ringer bicarbonate buffer, pH 7.4, in 95% 02-5% CO2 gas phase. A major product was proved as testosterone by means of paper and gas chromatographic analyses and by the derivative formation. Human chorionic gonadotropin (50 or 100 U in 1 flask) was shown to have a minor influence on the conversion of 4ADL to testosterone by rat testis in vitro.
Androst-5-ene-3β, 17β-diol (5ADL) was also converted to testosterone in the same rate as observed for 4ADL. Conversion of these 2 androstenedioles to androst-4-ene-3, 17-dione (4ADN) was observed to very minor extent compared to the conversion of dehydroepiandrosterone (DHEA) to 4ADN.
4-C14-DHEA was incubated with rat testis tissue preparation for 2 hrs. and 8 radioactive compounds converted from DHEA were separated on paper by extensive chromatography. Among these compounds, testosterone and 4ADN were identified by recrystallization with carrier steroid to constant specific activity. A compound behaving very similarly to the reference 4ADL on papers was separated. Oxidation of this compound with chromium trioxide yielded 2 products which showed the same mobilities as 4ADN and testosterone. An aliquot of the compound was added with carrier 4ADL and recrystallization showed the nearly constant specific activity. Testosterone zone derived from the compound by oxidation with chromium trioxide was added with carrier testosterone for recrystallization and the constant specific activity was demonstrated.
