Endocrinologia Japonica
Online ISSN : 2185-6370
Print ISSN : 0013-7219
ISSN-L : 0013-7219
C-20-REDUCED METABOLITES OF CORTISOL IN NORMAL HUMAN URINE
YOSHITO ITO
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ジャーナル フリー

1968 年 15 巻 1 号 p. 13-19

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抄録
Regular occurrence of Reichstein's substances E and U as free steroids in urine of normal subjects receiving neither exogenous cortisol nor ACTH, is demonstrated autoradiographically on thin-layer chromatograms after the intravenous administration of tracer doses of 4-14C-cortisol. A relatively simple method for the determination of these steroids as well as cortols and cortolones using periodic acid oxidation and thin-layer chromatographic separation is described. Mean normal values without correction were: Reichstein's substance E 140.3μg, Reichstein's substance U 122.4μg, cortols 431.0μg and cortolones 1156.0μg per 24 hrs.
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© The Japan Endocrine Society
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