Abstract
A new modification of the ovarian ascorbic acid depletion assay has been devised using adult pseudopregnant rats of the Wistar strain. The sample to be assessed for LH activity is injected in a volume of 0.005ml, directly into the ovarian bursa. Minimum effective doses of HCG and LH (NIH-LH-S5) averaged 0.005 I. U. and 0.005μg, respectively. Indices of precision were (lambdas) 0.18 for HCG and 0.12 for LH. The principal shortcoming lies in the fact that LH concentrations less than 1μg/ml cannot be detected, because of the volume restriction. This method, however, is useful particularly if only a small amount of material is available for assay.
