抄録
Biosynthesis of chondroitin sulfate in chick embryo femora was studied in tissue culture in a fundamental study to examine the effects of hormones on chondroitin sulfate metabolism in cartilagenous bones. The bones from 9-days old embryos were cultivated by the roller-tube method with various natural and synthetic media containing 35S-sulfate. The bones incorporated radiosulfate actively into chondroitin sulfate of their cartilage matrix. Total radioactivity in the bone was confirmed to reflect the net amount of chondroitin sulfate synthesized during cultivation, since the radiosulfate incorporating activity remained at the same high level and the biological decay of the labeled polysaccharide was very limited throughout the culture period. By measuring the radiosulfate uptake, therefore, the bones growing in natural or synthetic media were compared for their chondroitin sulfate synthesizing activity. And the biosynthesis of the sulfated polysaccharide was clearly shown to be dependent upon culture medium: e. g. a synthetic medium widely used in tissue culture, TC-199, was inferior to the natural medium which is capable of permitting physiologic chondrification of the bones but a chemically defined medium devised for bone cultivation, BGJb-HW, was comparable with the natural medium.
