抄録
In order to improve reproducibility of a quantitative hemagglutination inhibition for Human Chorionic Gonadotropin, effects of bovine serum albumin, normal rabbit serum and nonpregnant urine on the sensitivity of this test have been studied. As concentration of BSA in a diluent increases up to 1%, the sensitivity increases proportionally. Effect of 2% NRS is comparable to that of 0.1% BSA. Effect of nonpregnant urine on the sensitivity of the test is varied from one individual to another. mean effect of urine, however, nearly equals that of 0.1% BSA. From the comparison of a direct and a serial dilution method, and of dilution in cool and at temperature, it is suggested that the sensitivity increasing effect of protein could ascribed to its protective action to HCG molecules. The use of protein solution to dissolve standard HCG as well as urine samples is recommended for a quantitative or semi-quantitative assay of HCG in urine.
