Abstract
Two different gonadotropic fractions were obtained by the ion exchange chromatography on carboxymethyl cellulose and DEAE cellulose columns, following to the gel filtration through Sephadex G-100 of chicken adenohypophysial glycoprotein. Oneof the fractions possessed a high activity (3.4×NIH-FSH-S8) of follicle stimulating hormone and undetectable activity of luteinizing hormone. The other fraction possessed a low but significant activity (0.085×NIH-LH-S16) of luteinizing hormone and relatively low activity (0.59×NIH-FSH-S8) of follicle stimulating hormone. Gonadotropic activities assessed by chick testes 32P uptake method of the former and latter fractions were 1.6×and 3.0×NIH-LH-S16, respectively.
