The Simultaneous Assay of Progesterone, 17-Hydroxyprogesterone, and Deoxycorticosterone in Human Plasma by Competitive Protein Binding
1976 Volume 23 Issue 3 Pages 259-264
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1976 Volume 23 Issue 3 Pages 259-264
A method is described for the simultaneous measurement of prosterone (P), 17-hydroxyprogesterone (17-OHP) and deoxycorticosterone (DOC) in plasma by competitive protein-binding assay. After extraction, these steroids were purified by Sephadex LH-20 columns. The method described here is sensitive enough to detect the abovementioned steroids in as little as 5ml of plasma under physiological conditions. The mean physiological plasma level (ng/100 ml) of P, 17-OHP and DOC are 18.2±1.6 (SE), 136.5±11.7, and 8.6±1.0 in men, 14.7±1.7, 61.2±6.9 and 6.0±1.2 in women in the follicular phase, and 645.0-1-108.1, 155.2-1-16.9, and 7.5-F1.2, in the luteal phase of the menstrual cycle.
By this method and others, the responses of plasma levels of 7 adrenal steroids (P, 17-OHP, DOC, corticosterone, deoxycortisol, cortisol and aldosterone) to ACTH infusion (25U i. v. over 8 hours) were investigated in healthy men. The increase of DOC was more pronounced than that of the other steroids.
