Endocrinologia Japonica
Online ISSN : 2185-6370
Print ISSN : 0013-7219
ISSN-L : 0013-7219
Volume 23, Issue 3
Displaying 1-14 of 14 articles from this issue
  • TORU SUGASE, KYOHEI NONAKA, TAKASHI YOSHIDA, SUZUE MORISHITA, HIROYUKI ...
    1976 Volume 23 Issue 3 Pages 187-193
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Ten healthy male volunteers were studied to compare the effectiveness of intravenous and subcutaneous injections of 1mg of glucagon on HGH secretion. Plasma HGH level rose to a peak of 6ng/ml or greater 120 minutes after the subcutaneous injection of glucagon (sc glucagon) in all subjects, whereas the intravenous injection of glucagon (iv glucagon) caused comparable increments in plasma HGH in only six out of ten subjects. Furthermore, in comparison to those in sc glucagon the periods required to show maximum responses were less consistent in iv elucazon. Plasma IRG levels reached a peak of 102.4±22.6ng/ml at two minutes following iv glucagon, and a peak of 3.33±1.08ng/ml at 15 minutes following sc glucagon. These fell to initial levels at 60 minutes and at 180 minutes, respectively. There was no definite correlation either between the magnitudes of changes in plasma IRG and HGH levels or between the velocities of decrement in blood sugar and HGH responsiveness. Judging from its simplicity and reproducibility it may be concluded that sc glucagon is more suitable for a clinical provocative test of HGH release than is iv glucagon. In regards to the mechanism of glucagoninduced HGH release, neither glucagon per se nor the fall of blood sugar after hyperglycemia was assumed to play any major role. The sustained elevation of plasma IRG for a certain period might be responsible for the glucagon-induced HGH release.
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  • MASANOBU HONDA
    1976 Volume 23 Issue 3 Pages 195-202
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Plasma concentrations of progesterone (P), deoxycorticosterone (DOC), 17-hydroxyprogesterone (17-OH P), corticosterone (B), deoxycortisol (S), cortisol (F) and aldosterone (A) in 8 control subjects (mean age: 40.5 years) and 10 patients with essential hypertension (EH)(mean age: 48.5 years) were determined before, 4 and 8 hours after an infusion of ACTH at a rate of 25 units per 8 hours. Secretion rates (SR) of 18-hydroxy-11-deoxycorticosterone (18-OH DOC) were measured 24 hours before and again on the day of ACTH infusion. All subjects were studied on the fourth day of a diet containing 135 mEq of sodium and 90 mEq of potassium. There was no statistically significant difference between 8 control subjects and 10 patients with FH in the 7 plasma steroid levels and the SR of 18-OH DOC before ACTH infusion. The mean plasma presponse to ACTH was slightly lower in controls than in patients with EH, while that of 17-OH p (in male subjects) was slightly higher. The mean plasma B response was significantly lower atter 4 nours or At., in infusion (p<0.01), while that of DOC was significantly higher after 8 hours of ACTH infusion (p<0.05) in patients with EH. The mean plasma S rose significantly more in patients with EH (p<0.025) at 4 and 8 hours after ACTH infusion. The mean plasma F response to ACTH infusion was slightly lower in patients with EH than in controls. The mean response of 18-OH DOC SR to ACTH infusion was slightly higher in patients with EH than in controls. The mean plasma A response was significantly higher in patients with EH than in controls 4 (p<0.05) and 8 hours (p<0.001) after an ACTH infusion.
    These results could be explained in part by abnormalities in the 17-and 11-hydroxylase systems, and that the abnormality in 11-hydroxylation was more pronounced than that in the 17-position.
    Furthermore, we suspect that the sensitivity of adrenal aldosterone to ACTH might be increased or another accelerated pathway to aldosterone biosynthesis might exist in patients with EH.
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  • TOSHIHIKO ISHIDA, JIRO TAKAHARA, KOICHI KAWANISHI, KO KAWAMURA, AKIO G ...
    1976 Volume 23 Issue 3 Pages 203-206
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Effect of somatostatin on plasma gastrin levels was investigated in anesthetized dogs. Somatostatin was infused into the right gastroepiploic artery and plasma gastrin levels in the right gastroepiploic, superior pancreaticoduodenal and peripheral veins were measured by radioimmnuoassay. Somatostatin suppressed basal gastrin level promptly in the right gastroepiploic vein and impaired the normal gastrin secretion in response to intravenous infusion of arginine. The inhibitory effect of somatostatin on the plasma gastrin secretion was quite clearly demonstrated in the right gastroepiploic vein. It appears likely that the inhibition of gastrin secretion by somatostatin resulted in direct action on the gastrin secretory cell.
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  • YUKIO OCHI, TAKASHI HACHIYA, MANABU YOSHIMURA, TADAYOSHI MIYAZAKI, TAK ...
    1976 Volume 23 Issue 3 Pages 207-213
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    This study was undertaken to determine T3 content in red cells by radioimmunoassay. T3 in red blood cells was solubilized fairly from the stroma by hemolysis and red-cell T3 content could be determined directly by radioimmunoassay of the lysate. After hemolysing red cells with an equal volume of distilled water, 0.4ml of the hemolyzate was used for the assay. The red-cell T3 content was expressed as ng/ml of red-cell volume. The normal T3 range in red cells was 0.20-0.45ng/ml, and the Mean ±SD was 0.32±0.10ng/ml. The limit of detectability was 0.2ng/ml. In hyperthyroid patients, the red-cell T3 content was more than 0.50ng/ml with a Mean±SD of 1.35±0.65ng/ml. In hypothyroid patients, red cells contained less than 0.25ng/ml of T3, and there was an overlap from 0.20 to 0.25ng/ml in the content of red-cell T3 in hypothyroid and euthyroid subjects. The patients with T3 toxicosis showed a high or normal level of red-cell T3. A positive correlation was noted between the red-cell T3 content and the serum T3 level (r=0.66). The correlation between the red-cell T3 content and the free T4 index (expressed as T7) was also positive (r=0.67).
    From these experiments, it is suggested that the red-cell T3 is low in comparison with the serum T3 levels, and depends on two factors; serum T4 and serum T3 levels.
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  • REIKO DEMURA, HIROSHI DEMURA, KAZUO SHIZUME, OSAMI KUBO, KOICHI KITAMU ...
    1976 Volume 23 Issue 3 Pages 215-219
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    A female case of precocious puberty associated with HCG-producing ectopic pinealoma was reported. The patient, a 5-year-old girl, was referred to the hospital because of headache and choked discs. Physical examination revealed normal physical growth with breast enlargement. Endocrinological study revealed a high plasma HCG concentration of 1192ng/ml with a normal FSH level. None of HCG, LH and FSH did respond to the LH-RH test. A partial resection of the tumor and an external X-ray irradiation relieved the symptoms and breast enlargement subsided with a remarkable decrease in the plasma HCG level. Histological examination revealed two-cell-pattern pinealoma and electron microscopic findings showed abundant secretory granules in the dark cells. HCG content in the tumor was as high as 400ng/mg of acetone dried tumor tissue, but no FSH was detectable. Hitherto, all of the reported cases of precocious puberty associated with pineal tumors have been exclusively boys. A normal level of plasma FSH concentration with a somewhat elevated prolactin level might be a contributory factor for the development of precocial sexual development in the present case.
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  • JUN KAWADA, YOSHIYUKI YOSHIMURA, TAKESHI MINAMI
    1976 Volume 23 Issue 3 Pages 221-225
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Poly L-lysine, poly L-ornithine, and histone significantly inhibited the iodide uptake by the thyroid slices, as previously reported. These basic polymers diminished Na, K-ATPase and concomitantly markedly elevated Mg-ATPase activity in the Nal-treated microsomal preparation and the plasma membrane fraction obtained from thyroid. Poly L-glutamic acid, which was noneffetive to the iodide uptake in vitro, did not show such phenomenon. K-dependent p-nitrophenylphosphatase activity which is considered to reflect the terminal step of the reaction sequence of Na, K-ATPase was also inhibited by poly L-lysine. The effects mentioned above of poly L-lysine and other basic polyamino acids on membrane ATPase system were only found in the preparations from thyroid. The inhibitory effect of these reagents on thyroidal iodide uptake was discussed in terms of the change in membrane ATPase activities.
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  • SHINPEI MORIMOTO, KENZO UCHIDA, ISAMU MIYAMORI, IKUO YAMAMOTO, KYUNG S ...
    1976 Volume 23 Issue 3 Pages 227-231
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    The effects of treatment with a spironolactone derivative (SC 14266) for 9 experimental weeks on blood pressure and plasma renin activity (PRA) were investigated in unilaterally nephroadrenalectomized rats drinking 1% saline (“unilateral nephrectomy”), unilaterally nephroadrenalectomized and contralaterally adrenal-enucleated rats drinking 1% saline (“adrenal enucleation”) and intact rats drinking tap water (“normal”).
    The development of hypertension in “adrenal enucleation” rats was prevented by treatment with SC 14266 and the drug did not significantly affect the blood pressure in either “unilateral nephrectomy” or “normal” rats. SC 14266 did not influence a low level of basal PRA and the blunted response of PRA to furosemide administration in either “unilateral nephrectomy” or “adrenal enucleation” rats. On the other hand, PRA after furosemide administration in “normal” rats receiving SC 14266 was significantly higher than that in those rats treated with vehicle.
    The results suggest that the mineralocorticoid (s) secreted by the enucleated adrenal has a hypertensogenic property but no effect on the suppression of renin secretion under a high sodium intake and the unilateral nephrectomy.
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  • TAIZO UEMURA, NOBUO HANASAKI, SABURO YANO, YUICHI YAMAMURA
    1976 Volume 23 Issue 3 Pages 233-236
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    The bioactivity of ACTH released from isolated rat anterior pituitary glands into the incubation medium was determined.
    After the pituitaries were removed, ACTH activity in the medium decreased exponentially during further incubation at 37°C. The loss of ACTH activity was temperature-and pH-dependent and inhibited both by protease inhibitor (trasylol) and by preheating. Crude tissue extracts from median eminence, cerebral cortex and liver similarly inhibited the loss of ACTH activity.
    These results indicate that ACTH released into the medium may be destroyed by proteolytic enzyme (s) from the rat anterior pituitary.
    ACTH activity in the incubation medium was increased promptly by acidification of the medium to pH 1.5-2.5 with HC1, and reduced to the initial level by NaOH reneutralization of the medium (pH 6.8-7.8).
    These phenomena were not observed after the incubation medium had been heated at 100°C for 5 min.
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  • TSUGUO UEMURA, JIRO KOOGUCHI, SHOICHI TSUCHIHASHI, YOSHINORI SHIOJIMA
    1976 Volume 23 Issue 3 Pages 237-242
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    This paper describes the LH and FSH responses to synthetic LH-RH in 81 anovulatory women and 37 ovulatory women at various estradiol (Ed) levels. Besides, effects of mestranol on releases of LH and FSH in response to LH-RH were examined in 4 anovulatory women. Subjects were divided into 3 groups: low Ed group, moderate Ed group and high Ed group.
    No significant correlation was found in ovulatory women, but in anovulatory women a positive significant correlation was found between the LH/FSH ratio after LH-RH injection and the circulating Ed level. In ovulatory women, a mean LH/FSH after administration of LH-RH was not signifiocantly different from LH/FSH at the LH peak in the normal menstrual cycle. But in anovulatory women, a mean LH/FSH after the adminitration in the low Ed group was signifiocantly lower than LH/FSH and mean LH/FSH after the administration in two other Ed groups were significantly higher than that at the LH peak in the normal menstrual cycle. Among the low Ed group, four patients with primary ovarian failure and elevated gonadotropin levels in serum were treated with mestranol. With increased doses of mestranol, the LH/FSH after administration of LH-RH was increased and reached to the same level as the normal ovulatory peak in these 4 patients.
    Our results demonstrated that responses of pituitary gonadotropins to LH-RH in anovulatory women were significantly different in the LH/FSH from those in ovulatory women. Abnormality of these responses in anovulatory women seems to be unfavorable to ovulation.
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  • KOZO HASHIMOTO, JIRO TAKAHARA, HIDEMI HOSOGI, SHO YUNOKI, TADASHI OFUJ ...
    1976 Volume 23 Issue 3 Pages 243-249
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    The plasma ACTH responses to hydrocortisone infusion were compared in patients with Cushing's disease and primary adrenocortical insufficiency. In 4 patients with primary adrenocortical insufficiency, plasma ACTH levels were suppressed exponentially after administration of a relatively large dose of hydrocortisone (1.0 mg/kg/1.5 hr-3.0mg/kg/2 hr). In patients with post-adrenalectomized Cushing's disease (4, bilateral; 1, unilateral), plasma ACTH suppression was delayed. Plasma ACTH levels, expressed as a percentage of the basal concentrations, were significantly less suppressed in patients with Cushing's disease than in patients with primary adrenocortical insufficiency 90 (p<0.05) and 120 (p<0.05) min after the beginning of infusion. When 0.5mg/kg hydrocortisone was infused over a period of 1.5 hr, suppression was also delayed in Cushing's disease, and plasma ACTH levels were less suppressed in 4 patients with Cushing's disease than in 4 patients with primary adrenocortical insufficiency at 30 (p>0.05), 45 (p>0.05), 60 (p<0.01) and 90 (p<0.05) min.
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  • KANJI SATO, YUKITAKA MIYACHI, AKIRA MIZUCHI, NAKAAKI OHSAWA, KINORI KO ...
    1976 Volume 23 Issue 3 Pages 251-257
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    A highly specific and simple radioimmunoassay for cyclic AMP with a sensitivity of 0.04 picomoles/tube has been developed according to the method of Steiner et al., using 125I-succinyl cyclic AMP tyrosine methyl ester as a tracer. The tracer with higher immunoactivities could be simply and constantly prepared by an enzymatic iodination procedure utilizing lactoperoxidase, radioactive iodide and hydrogen peroxide generated by glucose-glucose oxidase system, rather than by chloramine-T procedure.
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  • MASANOBU HONDA
    1976 Volume 23 Issue 3 Pages 259-264
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    A method is described for the simultaneous measurement of prosterone (P), 17-hydroxyprogesterone (17-OHP) and deoxycorticosterone (DOC) in plasma by competitive protein-binding assay. After extraction, these steroids were purified by Sephadex LH-20 columns. The method described here is sensitive enough to detect the abovementioned steroids in as little as 5ml of plasma under physiological conditions. The mean physiological plasma level (ng/100 ml) of P, 17-OHP and DOC are 18.2±1.6 (SE), 136.5±11.7, and 8.6±1.0 in men, 14.7±1.7, 61.2±6.9 and 6.0±1.2 in women in the follicular phase, and 645.0-1-108.1, 155.2-1-16.9, and 7.5-F1.2, in the luteal phase of the menstrual cycle.
    By this method and others, the responses of plasma levels of 7 adrenal steroids (P, 17-OHP, DOC, corticosterone, deoxycortisol, cortisol and aldosterone) to ACTH infusion (25U i. v. over 8 hours) were investigated in healthy men. The increase of DOC was more pronounced than that of the other steroids.
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  • KAZUTOSHI OKANO, RUMIKO NAKAI, HAJIME GOTO, MASAKI YOSHIKAWA
    1976 Volume 23 Issue 3 Pages 265-269
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    Competitive protein-binding assay of 25-OH-D was developed by the use of specific vitamin D-binding proteins from vitamin D-deficient rat serum. Ether extract of serum sample which was dried and dissolved in ethanol, or standard solution of 25-OH-D3, was incubated with 3H-25-OH-D3 and vitamin D-binding protein for 2 hours at 4°C. Free and bound 3H-25-OH-D3 were separated through dextran-coated charcoal. The sensitivity of the assay system was 0.22 ng/tube. Percent cross reaction in the assay was 2.18% in vitamin D3, 0.70% in 1, 25-(OH) 2-D3, less than 0.28% in la-OH-D3, and less than 0.06% in dihydrotachysterol, cholesterol and cortisol.
    Human serum 25-OH-D is 28.9±2.9 ng/ml in 19 normal subjects. Serum 25-OH-D in the old-age group (50-70 years of age) was significantly decreased, compared with that in the young-age group (20-40 years of age). Serum 25-OH-D was significantly decreased in gastrectomized and osteoporotic patients as well as in the patients with liver cirrhosis, in comparison with their age-controls.
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  • HIROKO YASUDA, SEIJI SUZUKI, KAMEJIRO YAMASHITA, TOSHIO KANEKO, HIROSH ...
    1976 Volume 23 Issue 3 Pages 271-273
    Published: 1976
    Released on J-STAGE: January 25, 2011
    JOURNAL FREE ACCESS
    The effect of synthetic rat C-peptide on glucose-induced insulin release was studied in isolated islets of rat pancreas. Addition of rat C-peptide II to the medium inhibited glucose-induced insulin release from islets by 30 to 40 percent. Rat C-peptide I, human connecting peptide and bovine, porcine and canine C-peptides had no significant effects on insulin release from islets of rat pancreas.
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