Production of Anti-Human Thyroglobulin and Anti-Thyroid Hormone Antibodies in Rabbits Immunized with Human Thyroglobulin

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Two rabbits (TG-1, TG-2) were immunized with human thyroglobulin (HTg) and bled serially. Antisera were obtained at different times after the first immunization and kept separately and studied. In both rabbits production of anti-HTg, and anti-thyroid hormone antibodies such as anti-thyroxine (T4) and anti-triiodothyronine (T3) antibodies was observed. Binding parameters of anti-HTg antibodies with HTg, T4, and T3 were calculated in two selected antisera (70-day and 249-day). The Scatchard's plots of these antibodies were all curvelinear and were analyzed in two components: one, higher binding constant (Kal) and smaller binding capacity (Cap1) and the other, lower binding constant (Ka2) and larger binding capacity (Cap2). Kal values of anti-HTg, anti-T4, and anti-T3 antibodies in sera from TG-1 obtained from 70-day and 249-day bleeding were 1.1×10¹⁰M^-1, 6.0×10⁹ M^-1. 7.9×10⁸M^-1 and 1.7×10¹⁰M-1, 6.5 × 10⁹M^-1, 1.0 × 10⁹M^-1, respectively. Those from TG-2 were 1.7×10¹⁰M^-1, 1.8×10⁹M^-1, 6.4 × 10⁸M^-1 and 2.0×10¹⁰M^-1, 3.1×10⁹M^-1, 1.6×10⁹M^-1, respectively.
The significance of the production of anti-HTg and anti-thyroid hormone antibodies in rabbits immunized with HTg in relation to the antigenic structure of HTg molecule was discussed.