Responses of Plasma Adrenocorticotropin and Cortisol to Intravenous Injection of Synthetic Ovine Corticotropin Releasing Factor in the Morning and Early Evening in Normal Human Subjects

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This study was designed to compare the responsiveness of adrenocorticotropin (ACTH) and cortisol secretion to corticotropin-releasing factor (CRF) in the morning and early evening in normal human subjects. Synthetic ovine CRF (1.0μg/kg) or normal saline, was administered as an i.v. bolus injection to six normal males at 900h and 1700h. Blood samples were obtained before and 15, 30, 60, 90 and 120min after CRF or saline injection. Significant increases in plasma ACTH and cortisol levels were observed in all subjects at the both time of testing after CRF injection. The net increments in the areas under the concentration curve (areas in the CRF experiment minus those in the saline control experiment) were not statistically different for both ACTH (mean ±SEM: 41.0±10.6pg/mlh in the morning: 51.1±8.9pg/mlh in the evening) and cortisol (mean±SEM: 28.5±5.07μg/dl h in the morning; 36.2±4.0μ9/dl h in the evening). Also no significant diffbrence was observed in net increment, peak level and the ratio of peak level to the basal level of ACTH and cortisol after CRF injection. There were no appreciable changes in plasma concentrations of growth hormone, thyroid-stimulating hormone or prolactin, although slight but statistically significant rises in plasma levels of luteinizing hormone and follicle-stimulating hormone were observed.
These results suggest that there is no significant difference in responsiveness of the pituitary-adrenal axis to CRF in the morning (900h) and early evening (1700h), and thus the time of day will not necessarily have to be considered when CRF is used between these times in a clinical test to evaluate pituitary ACTH reserve.