Effect of Media Conditioned by Concanavalin A Activated Spleen Cells on Pancreatic Islet Cells

MANABU SAWADA; KOJI NAKANO; GOJI HASEGAWA; JUNKO SETOGUCHI; HIROFUMI SHIGETA; NAOTO NAKAMURA; TAKAHIRO KANATSUNA; MOTOHARU KONDO

doi:10.1507/endocrj1954.38.47

MANABU SAWADA, KOJI NAKANO, GOJI HASEGAWA, JUNKO SETOGUCHI, HIROFUMI SHIGETA, NAOTO NAKAMURA, TAKAHIRO KANATSUNA, MOTOHARU KONDO

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ジャーナルフリー

1991 年 38 巻 1 号 p. 47-52

DOI https://doi.org/10.1507/endocrj1954.38.47

詳細

抄録

The effect of media conditioned by concanavalin A-activated spleen cells (C-sup) on insulin release and its islet cell cytotoxicity were studied. In a functional study, C-sup significantly inhibited both basal insulin release and glucose-stimulated insulin release. Morphologically, C-sup had a destructive effect on isolated islets after 72h incubation. Islet cell cytotoxicity was shown by lactate dehydrogenase (LDH) release assay after 5 days incubation with C-sup in a dose-dependent manner. These results suggest that acceleration of the onset of diabetes in young diabetes prone (DP) Bio-Breeding/Worcester (BB/W) rats following the injection of C-sup may depend on the suppressive and cytotoxic effects of C-sup on pancreatic islet cells.

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