Abstract
By the means of isoelectric precipitation, the crude U. P. which contains a constant potency of 2mg/kg was prepared from human urine, and this crude U. P. can be purified with ethanol fractionation to be a potential substance having two-fold activity and its recovery is almost 100%. According to the pattern of electrophoresis, this purified U. P. seemed to be a homogeneous but yet, it is considered that this substance may contain any other component, because further purifying effect was observed by ammonium sulfate fractionation. The further purification of purified U. P. by the method of ammonium sulfate fractionation is not yet enough, whereas some increase of the recovery of activity may be expected, so that the application of a more excellent purifying method must be sought for it.
