Abstract
U. P. was purified easily by alumina column chromatography to obtain the highly purified preparation in good yield. In human U. P., a preparation whose activity was 100γ per kg, was obtained with the yield of 1.0-1.2mg per 1. and in rabbit U. P., a preparation was obtained, whose estimated values were 100γ per kg as the activity, and 5-6mg per 1. as the yield. It was, however, observed that even with the most purified active preparation of human U. P., the chemical homogeneity was not obtained; slight contamination with any other component was observed with ultracentrifugical pattern. The isoelectric point of human U. P. was estimated as pH 2.3 by using buffer solution (μ=0.5), and acid-proteinlike properties were observed.
