Detection of Nucleoprotein Gene of Sendai Virus in the Lungs of Rats by Touchdown Nested Reverse Transcription Polymerase Chain Reaction

Yukiharu HAYASE; Kiyokake TOBITA; Michisato KII; Youji HAKAMADA; Takio ARAI

doi:10.1538/expanim.46.307

Experimental Animals

Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124

Note

Detection of Nucleoprotein Gene of Sendai Virus in the Lungs of Rats by Touchdown Nested Reverse Transcription Polymerase Chain Reaction

Yukiharu HAYASE, Kiyokake TOBITA, Michisato KII, Youji HAKAMADA, Takio ARAI

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Keywords: hemagglutinating virus of Japan, laboratory rodents, paramyxovirus

JOURNAL FREE ACCESS

1997 Volume 46 Issue 4 Pages 307-310

DOI https://doi.org/10.1538/expanim.46.307

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Abstract

The nucleoprotein (NP) gene of Sendai virus was detected by touchdown nested reverse transcription polymerase chain reaction (RT-PCR) in the lungs of a rat presented with respiratory illness and high serum ELISA titer to Sendai virus. This method seemed to be of value in controlling infection in laboratory rodents.

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