Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
Note
Comparison of the Effects of Two Fixatives for Immunolocalization of Testosterone in theTestes of the Cynomolgus Monkey, Mouse and Rat
Ji-Hong LIANGTadashi SANKAITakashi YOSHIDAYasuhiro YOSHIKAWA
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ジャーナル フリー

2000 年 49 巻 4 号 p. 301-304

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We compared the effect of two fixatives, Bouin's fixative and neutralized buffered 4% formaldehyde (10% formalin), for immunolocalization of testosterone in the testes of cynomolgus monkeys, mice and rats. In the samples fixed with Bouin's fixative, immunoreactive testosterone was detected as intense deposits in the cytoplasm of Leydig cells of monkeys and mice. Immunoreactive testosterone was detected not only in Leydig cells of rats but also moderately shown within tubules. Immunoreactive testosterone could not be detected in the testes of monkeys, mice or rats fixed with neutralized buffered formalin because of the poor morphology caused by the fixative. It is concluded that Bouin's fixative is a suitable fixative for immunolocalization of testosterone in the testes of cynomolgus monkeys, mice and rats.

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© 2000 Japanese Association for Laboratory Animal Science
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