Differences in Responsiveness of Mouse Strain against p-Benzoquinone as Assessed by Non-Radioisotopic Murine Local Lymph Node Assay

Abstract

The non-radioisotopic modification of murine local lymph node assay (LLNA) by using 5-bromo-2'-deoxyuridine (BrdU) was conducted to investigate the strain-related difference of the responsiveness of mice to p-benzoquinone (PBQ) with BALB/cAnN, CBA/JN and CD-1 mouse strains. Strain and dose related differences were analyzed by two-way analysis of variance (two-way ANOVA). CBA/JN was considered to be the highest responsive strain to PBQ, and interaction was detected between CD-1 and each of the other inbred strains. These results support the recommendation in the OECD test guideline 429 and the skin sensitization test guideline of US-EPA with regard to the selection of mouse strain for LLNA.