Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 1341-1357
ISSN-L : 0007-5124
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Estrous Cycle Stage-Independent Treatment of PMSG and hCG can Induce Superovulation in Adult Wistar-Imamichi Rats
Hiroe KONAtsushi TOHEIRyoji HOKAOMotoo SHINODA
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JOURNAL FREE ACCESS

2005 Volume 54 Issue 2 Pages 185-187

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Abstract

The estrous cycle influence on the number of ovulated eggs after injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) was investigated in 12, 18, and 24 weeks old adult female Wistar-Imamichi (WI) rats. PMSG (150 IU/kg) was injected at metestrus, diestrus, proestrus, or estrus, followed by hCG (75 IU/kg) 55 h later. Ovulation was induced at all ages and stages of the estrous cycle. The number of ovulated eggs was not affected by stage for similarly aged rats, however, the number of ovulated eggs obtained after treatment decreased with age. These results demonstrate that the PMSG/hCG treatment can induce ovulation at any stage of estrous cycle in WI rats and efficient superovulation at 12 weeks of age.

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© 2005 Japanese Association for Laboratory Animal Science
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