Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of          Motility in Frozen and Thawed Rabbit Sperm

Kazutoshi Nishijima; Shinji Yamaguchi; Mai Tanaka; Yusuke Sakai; Chihiro Koshimoto; Masatoshi Morimoto; Teruo Watanabe; Jianglin Fan; Shuji Kitajima

doi:10.1538/expanim.63.149

Originals

Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm

Kazutoshi Nishijima, Shinji Yamaguchi, Mai Tanaka, Yusuke Sakai, Chihiro Koshimoto, Masatoshi Morimoto, Teruo Watanabe, Jianglin Fan, Shuji Kitajima

Author information

Kazutoshi Nishijima
Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
Shinji Yamaguchi
Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
Mai Tanaka
Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
Yusuke Sakai
Department of Bio-resources, Division of Biotechnology, Frontier Science Research Center, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan
Chihiro Koshimoto
Department of Bio-resources, Division of Biotechnology, Frontier Science Research Center, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan
Masatoshi Morimoto
Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
Teruo Watanabe
Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
Jianglin Fan
Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409-3898, Japan
Shuji Kitajima
Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan

Keywords: computer-assisted sperm analysis, cryopreservation, motility, rabbit, sperm

JOURNAL OPEN ACCESS

2014 Volume 63 Issue 2 Pages 149-154

DOI https://doi.org/10.1538/expanim.63.149

Details

Abstract

The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analysis system. Sperm motility with CLC supplementation was 29.4 ± 9.6% (mean ± SD), which was significantly higher than that of controls (20.8 ± 7.1%, P<0.05). The curvilinear velocity of sperm with CLC exceeded that of controls, whereas the values for linearity and wobble were significantly lower in sperm with CLC compared with controls. After artificial insemination, 44.3% of recovered ova were fertilized in the CLC-supplemented group, which was higher than the percentage in the control group (36.4%). The results indicate that supplementation with CLC improves the rate and quality of motility in rabbit sperm after freezing and thawing, and would be advantageous for successful cryopreservation.

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