Development of an effective microsatellite marker system to determine the          genetic structure of Meriones meridianus populations

Xin Liu; Xiuyi Yu; Yimei Xu; Xiaoyan Du; Xueyun Huo; Changlong Li; Jianyi Lv; Meng Guo; Jing Lu; Zhenwen Chen

doi:10.1538/expanim.19-0077

Original

Development of an effective microsatellite marker system to determine the genetic structure of Meriones meridianus populations

Xin Liu, Xiuyi Yu, Yimei Xu, Xiaoyan Du, Xueyun Huo, Changlong Li, Jianyi Lv, Meng Guo, Jing Lu, Zhenwen Chen

Author information

Xin Liu
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China
Xiuyi Yu
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China
Yimei Xu
Center for Laboratory Animal Research of Xinjiang, Center for Disease Control of Xinjiang, No. 138, Jianquan Yi Jie, Tianshan District, Urumqi City, Xinjiang Uygur Autonomous Region, 830011, P.R. China
Xiaoyan Du
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China
Xueyun Huo
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China
Changlong Li
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China
Jianyi Lv
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China
Meng Guo
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China
Jing Lu
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China
Zhenwen Chen
Department of Medical Genetics and Developmental Biology, Capital Medical University, No. 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, P.R. China

Keywords: biomarker, genetic diversity, Meriones meridianus, microsatellite loci

JOURNAL OPEN ACCESS
Supplementary material

2020 Volume 69 Issue 2 Pages 224-232

DOI https://doi.org/10.1538/expanim.19-0077

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Abstract

Understanding the genetic quality of the gerbil, Meriones meridianus, plays an important role in the study of medical biology. However, no effective system has been established for evaluating a population’s genetic diversity to date. In the present study, we established a set of reasonable evaluative systems based on microsatellite markers of the Mongolian gerbil by using the method of cross-amplification of species. Following electrophoresis analysis, short tandem repeat (STR) scanning, and sequencing, 11 microsatellite loci were identified by matching the criteria characteristics and were used to evaluate the genetic diversity of two stocks of Meriones meridianus: Meriones meridianus jei Wang, 1964 (M. m. jei) and Meriones meridianus cryptorhinus Blanford, 1875 (M. m. cryptorhinus) from Xinjiang, China. The microsatellite loci screened were highly polymorphic and were suitable for genetic quality control of Meriones meridianus. In addition, the quality of the non-bred M. m. jei and M. m. cryptorhinus strains in our study is sufficient for them to be promising stocks in the future for the farmed animal industry.

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