Abstract
The changes in the reactivity of the reproductive organs to human chorionic gonadotropin (H CG) accrding to age were studied in dd mice and inbred strains of mice.
The results obtained are summarized as follows:
1) Vagina was not opend in saline injccted control dd mice (dd) at 15 days of age; however, vaginal opening was observed in 42% of 12 day old dd injected with HCG
The hemorrhagic follicles and corpora lutea formation were obsevred non of control animals in dd at 27 days of age, but were positive (80%) in injected with HCG at 18 days of age.
The ovarian weight was increased from 12 to 21 days in both of controls and HCG injected mice, but the uterine weight was significantly increased to HOG at 12 days of age in comparison with saline injectcd controls
2) At the time of the injection, C3H/He mice (C3H/He) showed vaginal opening at 18 days of age, SS mice (SS) and dd at 24 days of age; however, in CF#1 mice (CF#1), DDK mice (DDK) and C57BL/6 mice (C57BL/6) vagina were not opend at 24 days of age.
The vaginal opening reaction to HCG at the time of autopsy showed a high positive rate at 15 days of age in all the strains of mice except DDK and dd.
The hemorrhagic follicles and corpora lutea were not formed at 24 days of age in the various inbred strains of mice.
These response to HOG was not induced in the mice at 15 days of age, but it developed at 21 days of age in all inbred strain of mice; and it was recognized that there were differences in the reactivity among the strains at 18 days of age.
The ovarian weight reaction to saline and HOG in each age was significantly different among the various strains of mice. That is, the rate of increase in ovarian weight to HOG at 24 days of age proved to be an increasing type for SS and decreasing types for DDK, C57BL/6, CF#1 and dd.
The uterine weight reaction to HOG was significantly different in strains and ages, the difference in strains being larger than that in ages.
3) As for the vaginal opening reaction to HOG, a dose of 1.5 I.U. at 12 days of age induced a maximal response in SS. The hemorrhagic f olicles and corpora lutea formation was not induced in SS to HOG at 18 days of age but positive response was induced to HOG at 21 days of age, and a significant difference in the response to HOG was noted between the doses of 1.5 I.U. and 6.0 I.U. But there was no significant difference in the response by doses of HOG at 24 days of age. The ovarian weight reaction in SS was increased at 21 days of age due to the HOG administration a significant difference by doses of HOG was recognized at 24 days of age. The uterine weight reaction due to doses of HOG was significantly different in SS at 12 days of age; however, the response to 6.0 I.U. of HOG showed a tendency to decrease at 24 days of age.
