抄録
In animal experiment, it is necessary that the factors which exist and might act an important role in the purpose of the experiment, should be studied initially according to the purpose of the experiment.
The authors have made an experiment to investigate whether the lethal dose of the snake venom for mice might be affected by the factors such as body weight, sexes of the mice and the route of the inoculation using five strains of mice derived from distinct sources.
As to the relationship between lethal doses and strains of mice, the response of gpc/Y for the venom was the same as that of dd/Y, and especially the strain gpc/Y was regareded as comgaratively homogeneous, since the responses of the strain were found quite similar in repeated observations.
There was no significant difference statistically between gpc/S and dd/S, and gpc/Y and dd/Y in the results obtained. But the susceptibility of the former group (gpc/S and dd/S) was slightly different from the latter, and also some tendency of fluctuation was noted among the results obtained from that group.
Toxicity for mice in connection with the body weight and sex of mice was as follows: LD50 of the venom for mice did not seem to be influenced by the body weight which was within the limit of 14 gram to 17' gram either in gpc/Y and gpc/S and no appreciable difference was seen between each sex as such size of mouse was employed.
However, the LD50 of the venom was affected remarkably by the route of the inoculation. Almost equal results were obtained either from intravenous or intraperitoneal inoculation, while mice resisted firmly to subcutaneous as well as to intramuscular inoculations. The subcutaneous and intramuscular inoculations are hardly regarded as highly accurate methods as their confidence limits of the LD50 are so wide. On the consideration of the results mentioned above, it may by concluded that the subcutaneous and intramuscular inoculation methods are inadequate to estimate the lethal dose of the snake venom for mice.
