抄録
Many spontaneous, chemical-induced, and radiation induced dominant white spot (W) and steel (Sl) mutation have been identified in the mouse. W and Sl mutations have been similar phenotypic effects including deficiencies in pigment cells, germ cells, hematopoietic stem cells, and mast cells. Numerous studies have suggested that W acts within the affected cell while Sl instead exerts its effects in the extracellular environment. Recently findings demonstrating that W encodes the c-kit prot-oncogene, a tyrosine kinase membrane receptor, have suggested that Sl encodes a ligand for c-kit. We studied the expression of c-kit mRNA in various tissues and cell lines of mouse. And then, we have partially purified c-kit ligand from conditioned medium of BALB /3T3 fibrobrasts. We investigated the effect for proliferation and differentiation to c-kit expressing cells, mast cell, Leydig cell by the partially purified ligand.
