Gene expression and characterization of aerotaxis transducer HemAT from  extremely halophilic archaeon Haloarcula japonica TR-1

Tadikara T; Matsubara T; Kubota Y; Kosaka T; Ozawa T; Tsukimura W; Yatsunami R; Fukui T; Nakasone K; Takashina T; Nakamura S

doi:10.3118/extremophiles.12.1_29

Gene expression and characterization of aerotaxis transducer HemAT from extremely halophilic archaeon Haloarcula japonica TR-1

Tadikara T, Matsubara T, Kubota Y, Kosaka T, Ozawa T, Tsukimura W, Yatsunami R, Fukui T, Nakasone K, Takashina T, Nakamura S

著者情報

ジャーナルオープンアクセス

2013 年 12 巻 1 号 p. 29-32

DOI https://doi.org/10.3118/extremophiles.12.1_29

詳細

抄録

An open reading frame encoding an aerotaxis transducer HemAT homolog (named HemAT-Hj) was found in the genome of extremely halophilic archaeon Haloarcula japonica TR-1. HemAT-Hj would be a cytoplasmic protein consisting of a sensor domain, a linker region and a methyl-accepting signaling domain. The hemAT-Hj gene was successfully expressed in Escherichia coli. Recombinant HemAT-Hj was purified and characterized. Absorption spectra showed that E. coli-produced HemAT-Hj contained a b-type heme molecule.

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