Toward in vitro reconstitution of self-replicating molecular systems

抄録

Recently, in vitro synthetic biology has been actively engaged in the reconstitution of artificial systems implemented with life-like characteristics to understand the principle and origins of life. One of the most important characteristics of life is self-reproducibility, which is originated from the self-regeneration ability of the gene replication and expression system (i.e., central dogma). In this review, we summarize recent progress in in vitro reconstitution of biological functions that constitute central dogma, DNA replication, transcription, and translation.