Evaluation of Bioactivity and Effect of Polymeric Stabilizers During Heat Treatment for theUnfolded Fraction of Human Epidermal Growth Factor

Akon Higuchi; Jen-Hao Lee; Yu Wang; Wen-Yih Chen; Qing-Dong Ling; Yung Chang

doi:10.2115/fiber.67.185

一般報文

Evaluation of Bioactivity and Effect of Polymeric Stabilizers During Heat Treatment for the Unfolded Fraction of Human Epidermal Growth Factor

Akon Higuchi ^†, Jen-Hao Lee ^†, Yu Wang ^†, Wen-Yih Chen ^†, Qing-Dong Ling ^†, Yung Chang ^†

著者情報

Akon Higuchi ^†
Department of Chemical and Materials Engineering, National Central University, , National Central University, No. 300, Jhongli, Taoyuan 32001, Taiwan
Department of Reproduction, National Research Institute for Child Health and Development, 2-10-1, Okura, Setagaya-ku, Tokyo 157-8535, Japan
Cathay Medical Research Institute, Cathay General Hospital, , No. 32, Ln 160, Jian-Cheng Road, Hsi-Chi City, Taipei 221, Taiwan
Jen-Hao Lee ^†
Department of Chemical and Materials Engineering, National Central University, , National Central University, No. 300, Jhongli, Taoyuan 32001, Taiwan
Yu Wang ^†
Department of Chemical and Materials Engineering, National Central University, , National Central University, No. 300, Jhongli, Taoyuan 32001, Taiwan
Wen-Yih Chen ^†
Department of Chemical and Materials Engineering, National Central University, , National Central University, No. 300, Jhongli, Taoyuan 32001, Taiwan
Qing-Dong Ling ^†
Cathay Medical Research Institute, Cathay General Hospital, , No. 32, Ln 160, Jian-Cheng Road, Hsi-Chi City, Taipei 221, Taiwan
Institute of Systems Biology and Bioinformatics, National Central University, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan
Yung Chang ^†
Department of Chemical Engineering, R&D Center for Membrane Technology , Chung Yuan Christian University, 200, Chung-Bei Rd., Chungli, Taoyuan 320, Taiwan
著者の貢献

†Akon Higuchi, Jen-Hao Lee, Yu Wang, Wen-Yih Chen, Qing-Dong Ling, Yung Changは本研究に等しく貢献した。

ジャーナルフリー

2011 年 67 巻 8 号 p. 185-191

DOI https://doi.org/10.2115/fiber.67.185

詳細

抄録

A method independent of the use of radioisotope monomers such as [ 3 H]-thymidine was developed to evaluate the bioactivity of human epidermal growth factor (hEGF). Briefly, DNA synthesis of fibroblasts in hEGF- containing culture medium was evaluated via cell cycle experiments using flow cytometry ; the DNA synthesis ratio was used to evaluate the bioactivity of hEGF. The unfolded fraction of hEGF, a physical parameter, was also evaluated by circular dichroism measurements. The hEGF with a higher unfolded fraction showed less bioactivity than hEGF from fibroblasts. There was a good relationship between the bioactivity of hEGF (i.e., DNA synthesis ratio) and the evaluated physical parameter of hEGF (i.e., unfolded fraction). The stability of hEGF solution containing several polymeric stabilizers during heat treatment was also determined by physical evaluation of the unfolded fraction and by bioactivity measurement of the DNA synthesis ratio. Heparin and a low molecular weight chemical of mannitol were effective stabilizers of hEGF during heat treatment.

責任著者(Corresponding author)

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