1995 年 61 巻 2 号 p. 181-185
Monoclonal antibody (mAb) YeT-2 was generated in mice hyper-immunized with thym c lympho-cytes of yellowtail Seriola quinqueradiata. The mAb YeT-2 reacted withthe major population of peripheral blood lymphocytes, but not with serum IgM. OnPercoll density gradientcentrifugation, YeT-2 positive cells were distributed at densities of 1.058 and 1.064. The latter density layer also included some YeT-2 negative cells. Furthermore, on electron microscopic studies, YeT-2 positivelymphocytes in peripheral blood could not be distinguished from YeT-2 positivelymphocytes in thymus. Flow cytometric analysis showed that mAb YeT-2 positivelymphocytes comprised 88.4% and 76.1% of the peripheral blood and thymus, respectively. Functional studies revealed that YeT-2 positive lymphocytes respond to concanavalin A but not to lipopolysaccharide, while YeT-2 negative lymphocytes responded in the opposite way. From these results, it can be concluded that the novel mAb YeT-2 recognizes a lymphocytic population which might be the putative T cell.
