Isoation and Characterization of Serum Lipoproteins in Red Sea Bream

抄録

The very-low-density lipoproteins (VLDL), low-density lipoproteins (LDL) and high-density lipoproteins (HDL) were isolated from the serum of red sea bream by sequential preparative ultracentrifugation and high performance gel-filtration chromatography. The major lipid components of VLDL and LDL were triglycerides; however, thepercentage of sterol esters in LDL was higher than that in VLDL. The HDL contained phospholipids as the major lipid components. Red sea beam apo VLDL and apo LDL contained predominantly apo B-like proteins (150-250 kDa) in addition to small molecular weight proteins(6-7 kDa). Apo A-I-like (27.5 kDa) and apo A-II-like proteins (14 kDa) were the major constituents of apo HDL. The isoelectric points of two major isoforms of apo A-I-like protein (pI 5.4 and 5.3) were similar to thoseof a human counterpart. The molecular weight of apo A-II-like protein was approximately twice as large as that of human apo A-II and the isoelectricpoints of its major isoforms (pI 5.3 and 5.2) were more basic than the major isoform (pI 5.0) of human apo A-II.