抄録
The induction of cytochrome P4501A (CYP1A) mRNA in red sea bream Pagrus major was investigated using CYP1A cDNA. The CYP1A cDNA was derived from a 3-methylcholanthrene (MC)-inducible mRNA of P. major. CYP1A mRNA was induced in the liver, gill, gut and kidney after an intraperitoneal injection of 3-MC, although the induction levels of mRNA were different among these organs. Results of the time course experiment showed that the CYP1A mRNA content rapidly increased immediately after the 3-MC injection, then showed a slight decrease prior to a further increase until at least 70h after the injection. The CYP1A mRNA content increased in a concentration-dependent manner when fish were treated with various concentrations of 3-MC. The effect of water temperature on the induction of CYP1A mRNA was examined. The level of mRNA content was approximately double in fish maintained at 15°C of sea water than those at 11°C and 19°C. However, the induction levels of CYP1A mRNA were still higher in fish at 11 and 19°C than in the control fish.
These results suggested that the CYP1A mRNA may be useful as a biological indicator of the presence and concentration of chemical pollutants.
