1995 年 61 巻 5 号 p. 787-790
Immunoglobulins in the oocytes, fertilized eggs, and yolk sac larvae of red sea bream were successfully detected by SDS-PAGE and Western blotting, using rabbitantibody against red sea bream blood IgM. Those immunoglobulins appeared structurally identical to the blood IgM of the mother fish, based on the same molecular weight of their H and L chains to those found for the blood IgM, 78 kDaand 26 kDa respectively. Referring to the oocytic immunoglobulin, this appeared in the early stage ofvitellogenesis before the spawning season and was also present in reproductively active ovaries during spawning. Those observations indicate that immunoglobulin existing in the mother's blood can be transferred to its oocytes and subsequently to eggs and yolk sac larvae in red sea bream.
