抄録
Full-length cDNA for a 71 kDa constitutively expressed heat-shock-cognate protein (HSC71), a member of the HSP70 family, was isolated from a Japanese flounder embryo cDNA library. It has a single ORF of 1950bp that encodes a protein of 71. 2 kDa. Japanese flounder HSC71 contains a EEVD (Glu-Glu-Val-Asp) peptide motif at the C-terminal end which is a common feature of cytosolic HSC proteins in the HSP70 family. Japanese flounder HSC71 is 96.0%, 93.9%, 93.5%, and 93.4% identical in primary structure to rainbow trout HSC71, bovine HSC71, rat HSC71, and human HSC71, respectively. These results suggest that the HSC71 amino acid sequence has been highly conserved among vertebrates. Northern blot analysis showed that HSC71 mRNA was expressed constitutively in the Japanese flounder cells in early passage regardless of heat shock treatment.
