cDNA Cloning of Polypeptide Chain Elongation Factor 1α from Medaka Oryzias latipes

Masato Kinoshita; Takahiro Nakata; Kousuke Adachi; Taijiro Yabe; Yoshihiro Yokoyama; Haruhiko Toyohara; Takashi Hirata; Eiji Takayama; Satoshi Mikawa; Noriyuki Kioka; Morihiko Sakaguchi

doi:10.2331/fishsci.65.133

cDNA Cloning of Polypeptide Chain Elongation Factor 1α from Medaka Oryzias latipes

Masato Kinoshita, Takahiro Nakata, Kousuke Adachi, Taijiro Yabe, Yoshihiro Yokoyama, Haruhiko Toyohara, Takashi Hirata, Eiji Takayama, Satoshi Mikawa, Noriyuki Kioka, Morihiko Sakaguchi

著者情報

キーワード: polypeptide elongation factor lα, EF1α, cDNA, medaka

ジャーナルフリー

1999 年 65 巻 1 号 p. 133-137

DOI https://doi.org/10.2331/fishsci.65.133

詳細

抄録

Polypeptide chain elongation factor 1α (EF1α) was cloned from a liver cDNA library of medaka Orizias latipes. The analysis of the clone containing 1.6kbp DNA revealed that the entire open reading frame of EFla was 1383 bp long. Deduced amino acid sequence of medaka EFlα showed high similarity (more than 87% identity) with those of other vertebrates. Medaka EFlα had one amino acid deletion at the position of 447 amino acid of other EF1αs and unique 17 replacement against those of other vertebrates. Northern hybridization analysis revealed that this EF1α was expressed in all tissues tested.

責任著者(Corresponding author)

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