Endocrine control of vitellogenesis in the Japanese scallop

MAKOTO OSADA; AKIHIRO KIJIMA

doi:10.2331/fishsci.68.sup1_704

MAKOTO OSADA, AKIHIRO KIJIMA

著者情報

キーワード: scallop, ovary, vitellin, vitellogenin

ジャーナルフリー

2002 年 68 巻 sup1 号 p. 704-707

DOI https://doi.org/10.2331/fishsci.68.sup1_704

詳細

Published: 2002 Received: - Available on J-STAGE: 2008/06/30 Accepted: - Advance online publication: - Revised: -
訂正情報
訂正日: 2008/06/30 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : The ELISA analysis with anti-scallop vitellin (Vn) serum demonstrated that an in vitro accumulation of Vn in the ovarian tissue was promoted with estradiol-17β and cerebral plus pedal ganglion (CPG) extract which was heat stable. Estrogen-like immunoreactivity was found in the growing oocyte and auxiliary cell. It was supposed that the vitellogenesis occurred inside the ovary and an oocyte growth appeared to be controled by estrogen and CPG via estrogen receptor (ER). Vitellogenin (Vtg) cDNA clone was isolated from the ovary by immunoscreening and the putative protein exhibited 20% of homology, compared with Vtgs of other oviparous animals. RNA blot-hybridization analysis revealed that over 10 kb of mRNA was detected only in the ovary and a high amount of Vtg mRNA during oocyte growth was retained until spawning stage. In situ hibrydization demonstrated Vtg mRNA expression in the auxiliary cells closely associated with growing oocytes. The content of mRNA in the ovarian tissue cultured in vitro with CPG showed no change although the CPG extract enhanced Vn accumulation. A transcription and translation of Vtg mRNA were supposed to be regulated by the different mechanisms, respectively.
Right : The ELISA analysis with anti-scallop vitellin (Vn) serum demonstrated that an in vitro accumulation of Vn in the ovarian tissue was promoted with estradiol-17ß and cerebral plus pedal ganglion (CPG) extract which was heat stable. Estrogen-like immunoreactivity was found in the growing oocyte and auxiliary cell. It was supposed that the vitellogenesis occurred inside the ovary and an oocyte growth appeared to be controled by estrogen and CPG via estrogen receptor (ER). Vitellogenin (Vtg) cDNA clone was isolated from the ovary by immunoscreening and the putative protein exhibited 20% of homology, compared with Vtgs of other oviparous animals. RNA blot-hybridization analysis revealed that over 10 kb of mRNA was detected only in the ovary and a high amount of Vtg mRNA during oocyte growth was retained until spawning stage. In situ hibrydization demonstrated Vtg mRNA expression in the auxiliary cells closely associated with growing oocytes. The content of mRNA in the ovarian tissue cultured in vitro with CPG showed no change although the CPG extract enhanced Vn accumulation. A transcription and translation of Vtg mRNA were supposed to be regulated by the different mechanisms, respectively.

訂正日: 2008/06/30 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1. Wahli W. Evolution and expression of vitellogenin genes. Trends in Genetics, 1988; 4: 227-232.
2. De jong-Brink M, Boer HH, Joosse J. Mollusca. In: Adiyodi KG, Adiyodi RG (eds). Reproductive Biology of Invertebrates Vol. 1. Wiley, Chichester, 1983; 297-335.
3. Barre P, Bride M, Beliard R, Petracca B. Localization of yolk proteins and their possible precursors using polyclonal and monoclonal antibodies, in Helix asperssa. Cell. Mol. Biol., 1991; 37: 639-650.
4. Bride M, Petracca B, Faivre D. The synthesis of vitellogenins by the digestive gland of Helix aspersa: Evidence from cell-free transcription of mRNA. Cell. Mol. Biol., 1992; 38: 181-187.
5. Li Q, Osada M, Suzuki T, Sato M, Mori K. Degradation of vitellin during embryonic and larval development in the Pacific oyster Crassostrea gigas. Invertebr. Reprod. Dev., 1998; 33: 1-9.
6. Suzuki T, Hara A, Yamaguchi K, Mori K. Purification and immunolocalization of a vitellin-like protein from the Pacific oyster Crassostrea gigas. Mar, Biol., 1992; 113: 239-245.
7. Osada M, Unuma T, Mori K. Purification and characterization of a yolk protein from the scallop ovary. Nippon Suisan Gakkaishi, 1992; 58: 2283-2289.
8. Matsumoto T, Osada M, Osawa Y, Mori K. Gonadal estrogen profile and immunohistochemical localization of steroidogenic enzymes in the oyster and scallop during sexual maturation. Comp. Biochem. Physiol., 1997; 1188: 811-817.
9. Li Q, Osada M, Suzuki T, Mori K. Changes in vitellin during oogenesis and effect of estradiol-17β on vitellogenesis in the Pacific oyster Crassostrea gigas. Invertebr. Reprod. Dev., 1998; 33: 87-93.
10. Osada M, Nakata A, Matsumoto T, Mori K. Pharmacological characterization of serotonin receptor in the oocyte membrane of bivalve molluscs and its formation during oogenesis. J. Exp. Zool., 1998; 281: 124-131.
11. Pipe RK. Oogenesis in the marine mussel Mytilus edulis: an ultrastructural study. Mar. Biol., 1987; 95: 405-414.
12. Gagné F, Blaise C, Salazar M, Salazar S, Hansen PD. Evaluation of estrogenic effects of municipal effluents to the freshwater mussel Elliptio complanata. Comp. Biochem. Physiol. C, 2001; 128: 213-225.
13. Eckelbarger KJ, Davis CV. Ultrastructure of the gonad and gametogenesis in the eastern oyster, Crassostrea virginica. I, Ovary and oogenesis. Mar. Biol., 1996; 127: 79-87.

Right : 1. Wahli W. Evolution and expression of vitellogenin genes. Trends in Genetics, 1988; 4: 227-232.
2. De jong-Brink M, Boer HH, Joosse J. Mollusca. In: Adiyodi KG, Adiyodi RG (eds). Reproductive Biology of Invertebrates Vol. 1. Wiley, Chichester, 1983; 297-335.
3. Barre P, Bride M, Beliard R, Petracca B. Localization of yolk proteins and their possible precursors using polyclonal and monoclonal antibodies, in Helix asperssa. Cell. Mol. Biol., 1991; 37: 639-650.
4. Bride M, Petracca B, Faivre D. The synthesis of vitellogenins by the digestive gland of Helix aspersa: Evidence from cell-free transcription of mRNA. Cell. Mol. Biol., 1992; 38: 181-187.
5. Li Q, Osada M, Suzuki T, Sato M, Mori K. Degradation of vitellin during embryonic and larval development in the Pacific oyster Crassostrea gigas. Invertebr. Reprod. Dev., 1998; 33: 1-9.
6. Suzuki T, Hara A, Yamaguchi K, Mori K. Purification and immunolocalization of a vitellin-like protein from the Pacific oyster Crassostrea gigas. Mar, Biol., 1992; 113: 239-245.
7. Osada M, Unuma T, Mori K. Purification and characterization of a yolk protein from the scallop ovary. Nippon Suisan Gakkaishi, 1992; 58: 2283-2289.
8. Matsumoto T, Osada M, Osawa Y, Mori K. Gonadal estrogen profile and immunohistochemical localization of steroidogenic enzymes in the oyster and scallop during sexual maturation. Comp. Biochem. Physiol., 1997; 118B: 811-817.
9. Li Q, Osada M, Suzuki T, Mori K. Changes in vitellin during oogenesis and effect of estradiol-17ß on vitellogenesis in the Pacific oyster Crassostrea gigas. Invertebr. Reprod. Dev., 1998; 33: 87-93.
10. Osada M, Nakata A, Matsumoto T, Mori K. Pharmacological characterization of serotonin receptor in the oocyte membrane of bivalve molluscs and its formation during oogenesis. J. Exp. Zool., 1998; 281: 124-131.
11. Pipe RK. Oogenesis in the marine mussel Mytilus edulis: an ultrastructural study. Mar. Biol., 1987; 95: 405-414.
12. Gagné F, Blaise C, Salazar M, Salazar S, Hansen PD. Evaluation of estrogenic effects of municipal effluents to the freshwater mussel Elliptio complanata. Comp. Biochem. Physiol. C, 2001; 128: 213-225.
13. Eckelbarger KJ, Davis CV. Ultrastructure of the gonad and gametogenesis in the eastern oyster, Crassostrea virginica. I, Ovary and oogenesis. Mar. Biol., 1996; 127: 79-87.

訂正日: 2008/06/30 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

The ELISA analysis with anti-scallop vitellin (Vn) serum demonstrated that an in vitro accumulation of Vn in the ovarian tissue was promoted with estradiol-17ß and cerebral plus pedal ganglion (CPG) extract which was heat stable. Estrogen-like immunoreactivity was found in the growing oocyte and auxiliary cell. It was supposed that the vitellogenesis occurred inside the ovary and an oocyte growth appeared to be controled by estrogen and CPG via estrogen receptor (ER). Vitellogenin (Vtg) cDNA clone was isolated from the ovary by immunoscreening and the putative protein exhibited 20% of homology, compared with Vtgs of other oviparous animals. RNA blot-hybridization analysis revealed that over 10 kb of mRNA was detected only in the ovary and a high amount of Vtg mRNA during oocyte growth was retained until spawning stage. In situ hibrydization demonstrated Vtg mRNA expression in the auxiliary cells closely associated with growing oocytes. The content of mRNA in the ovarian tissue cultured in vitro with CPG showed no change although the CPG extract enhanced Vn accumulation. A transcription and translation of Vtg mRNA were supposed to be regulated by the different mechanisms, respectively.

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