抄録
Opsonization is a crucial function of the complement system in immune clearance. To identify receptors responsible for the complement-mediated opsonization in teleost, head kidney leukocytes from β-1, 3-glucan-injected and non-injected common carp (Cyprinus carpio) were subjected to a rosette-forming assay with fixed sheep erythrocytes (Ef). Leukocyte fractions rich in granulocytes and macrophages from the glucan-injected carp had an ability to form the rosette with complement-coated Ef but the corresponding fractions from non-injected fish did not, suggesting that β-1, 3-glucan elicits expression of complement receptors on those cells. While sensitization of Ef with antibodies slightly elevated the rosette formation, complement-coated Ef showed a significant increase in rosette formation and this formation was inhibited with anti-carp C3. Rosette formation was also inhibited by EDTA but not by EGTA. These results suggest that complement-dependent opsonization in carp is mediated by C3-receptors analogous to mammalian complement receptor type 3 (CD11b/CD18 or integrin αM/β2), which recognizes the iC3b fragment in a divalent cation-dependent manner.
