Abstract
In previous reports, the author stated that glucose mobilization of toad's liver was influenced in accordance with the chemical property of the perfusing solution and also by seasonal change. In this present study, the author sought to determine the influence of the autonomic nervous system as well as that of the central nervous system on the glucouse mobilization of toad's liver through the electrical stimulation of these nervous systems.
The results of experiment were as follows :
1) Glucose mobilization was augmented by electrical stimulation of nervus splanchnicus. But this was not the case when perfused with yohimbin even though nervus splanchnicus was stimulated. 2) Glucose mobilization was enhanced by electrical stimulation of vago-sympathicus nerve.But this effect of stimulation was not affected when perfused with atropin previous to an electrical stimulation. 3) Glucose mobilization was not influenced by perfusion of yohimbin (1 : 100.000). 4) Glucose mobilization was augmented when perfused with atropin (1 : 100, 000). 5) Glucose mobilization did not fluctuated by electrical stimulation of the root of vagus nerve. 6) Glucose mobilization has been promoted by electrical stimulation on funiculus marginalis of the cervical sympathetic nerve, but, on the other hand, the effect of stimulation diminished by perfusing yohimbin solution before the electrical stimulation. 7) Glucose mobilization was not influenced by electrical stimulation of either Lobus olfactorius, Cortex cerebrum or Lobus opticus. 8) Glucose mobilization was found to be augmented by electrical stimulation of Fossa rhomboidea. 9) Glucose mobilization was increassed by electrical stimulation of Fossa rhomboidea even though after both sides of the Nn. splanchnici were cut, but it was not so remarkable as when the nerves were not cut. 10) Glucose mobilization was promoted by electrical stimulation of Fossa rohmboidea after both sides of the Nn. vago-sympathici were cut, but it was not so remarkable as when the nerves were still intact. 11) Glucose mobilization was augmented by electrical stimulation of Fossa rhomboidea after the upper portion above the after-brain was cut, and it shows almost the same value as when the after-brain was intact. 12) Glucose mobilization was not influenced when the liver was perfused with yohimbin even though the electrical stimulation of Fossa rhomboidea was additionally made. 13) The effect of the electrical stimulation of Fossa rhomboidea was not markedly influenced even though the liver was perfused with atropin. 14) Glucose mobilization was not influenced when the liver was perfused with acetylcholin (1 : 100, 000, 000-10, 000, 000). 15) Glucose mobilization was markedly augmented when the central nervous system was perfusea with strychnine (1 : 10, 000, 000-1, 000, 000) or picrotoxin (1 : 10, 000, 000-1, 000, 000). 16) Glucose mobilization was not influenced when the central nervous system was perfused with morphine (1 : 1, 000, 000, 000-100, 000) or urethane (1 : 1, 000-500) or ether (1 : 1, 000-500). By above experimental results, we reached to conclusions that the glucose mobilization of toad's liver can definitely be influenced by the autonomic nervous system as well as by the central nervous system, and that the effect of electrical stimulation of Fossa rhomboidea does not only belongs to the sympathetic nature, but also it may reach the liver through the N. splanchnicus and the cervical sympathetic nerves. [Cf. original (japanese) p. 259.]
