Abstract
Eggs treated with hyaluronidase were exposed to the Ringer·solution of digitoxigenin, lanatoside C, g-strophanthin, or digitoxin. The percentage or the time until onset of A-V block and percentage mortality 24 hours after exposure to the solution were observed to determine : 1) the relation between stages of development (myogenic and innervated), 2) influence of temperature (22°C, and 30°C), 3) interrelation of glycosides, 4) linear relation between the time until onset of A-V block and log concentration of digitalis, and 5) influence of ethanol and eserine on the action of digitalis glycosides. A-V block occurs faster and in higher percentage in innervated stage, at higher temperature, and in the order of digitalis preparations described above. By the linearity of the time concentration curve of digitalis, 10-10 g/cc concentration can be detected, and 1/2×10-9 g/cc determined quantitatively, using 0.02 cc of sample solution (containing 1 or 0.1% of ethanol) on each eserinized (10-9, 30 min) egg at 30°C.
