Fundamental Toxicological Sciences
Online ISSN : 2189-115X
ISSN-L : 2189-115X
Original Article
Effects of benzotriazole ultraviolet stabilizers on rat PXR, CAR and PPARα transcriptional activities
Yoko WatanabeShoko HattoriChieri FujinoKen TachibanaHiroyuki KojimaKouichi YoshinariShigeyuki Kitamura
ジャーナル フリー

2019 年 6 巻 2 号 p. 57-63


Benzotriazole ultraviolet stabilizers (BUVSs) are widely used as ultraviolet filters in various consumer and industrial products. For this purpose, we examined the effects of 10 BUVSs and benzotriazole itself on transcriptional activation mediated by nuclear receptors: pregnane X receptor (PXR), constitutive androstane receptor (CAR) and peroxisome proliferator-activated receptor alpha (PPARα). UV-090 and UV-9 showed rat PXR-agonistic activity in the concentration range of 1-30 μM in reporter gene assay using simian kidney COS-1 cells. UV-090 showed the highest activity (REC20 value: 3.85 × 10-6 M). UV-090 was also positive in rat CAR activation assay, while UV-P showed inverse agonistic activity towards CAR. In the presence of the CAR agonist artemisinin (10 μM), UV-P also showed dose-dependent CAR-antagonistic activity in the concentration range of 10-30 μM. UV-090 and UV-9 activated rat PPARα. Overall, these results suggest that UV-090, UV-9 and UV-P modulate PXR, CAR and/or PPARα activation.

© 2019 The Japanese Society of Toxicology