Fundamental Toxicological Sciences
Online ISSN : 2189-115X
ISSN-L : 2189-115X
最新号
選択された号の論文の3件中1~3を表示しています
Letter
  • Kazuyo Sadamoto, Shingo Nemoto, Akio Kawamura, Masaaki Kurata
    2019 年 6 巻 7 号 p. 245-248
    発行日: 2019年
    公開日: 2019/10/09
    ジャーナル フリー

    In the therapy of ocular diseases, different active pharmaceutical ingredients are often instilled concomitantly as different ophthalmic solutions at an appropriate interval (unfixed-combination therapy) or simultaneously as one formulation (fixed-combination therapy). In this study, we aimed to compare the in vitro cytotoxicity of fixed- and unfixed-combination ophthalmic solutions using the glaucoma therapeutic agents, timolol and brimonidine. Cultured human corneal epithelial cell line was used as a test system. Exposure period was set at 5 min. Compared with the fixed-combination treatment, the unfixed-combination treatment (timolol followed by brimonidine, and vice versa) led to a significant reduction in the percentage of cell viability. In conclusion, it was suggested that a fixed-combination ophthalmic solution was expected to cause less cellular damage to the ocular surface, compared to an unfixed-combination ophthalmic solution.

Letter
Letter
  • Atsushi Watanabe, Katsuhiro Miyajima, Noriko Kemuriyama, Hisashi Uchiy ...
    2019 年 6 巻 7 号 p. 253-258
    発行日: 2019年
    公開日: 2019/10/11
    ジャーナル フリー

    Ovariectomized rats were used as an animal model of osteoporosis in this study. They were given ad libitum access to a diet with high or low calcium to phosphorus ratio, or a normal diet, for a period of 10 weeks and effects on osteoporosis were assessed. Our results showed that the experimental diets affect urine levels of calcium and phosphorus, but do not affect the levels of biochemical, histopathological, or bone-related biomarkers. These results indicate that the administration of feed with different ratios of calcium to phosphorus (the phosphorus content was kept constant and the calcium content varied from 0.2× to 5× relative to the phosphorus content) over a period of 10 weeks does not accelerate the development of osteoporosis in ovariectomized rats with normal renal function to maintain homeostasis.

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