抄録
This is a report of observation of methylene blue, fat and iron absorbed into the human duodenal mucosa. Method: 0.5% methylene blue, iron (ferrous sulfate of saccharated ferric oxide combined with 0.1 N HCl and physiological sodium) or fat (salad oil and milk which were mixed and homogenized) was sprinkled on the duodenal mucosa through the fiberscope (GIF-D II, Olympus). Removal of these substances was done with water for washing several minutes later (0.5% methylene blue) or 20 minutes later (iron and fat) of their introduction. Biopsy specimens were stained with hematoxylin, prussian blue or Sudan 111 after formalin-fixation. Observation of biopsy specimens with and without staining was performed under the dissecting microscope. Histological study was also performed. Results: 1. Methylene blue. The normal mucosa was changed its odor into dark blue. Methylene blue solution within the villus tissue was detected under the dissecting microscope. Cryostat preparation of biopsy specimen revealed that methylene blue was taken into the cytoplasm of epithelial cells. Uptake of methylene blue was less effective in the circumference of duodenal ulcer, at erosive changes, island of gastric mucosa, some cases of white colored villi and small elevation due to protruberant lymph follicle. Hematoxylin staining made clearer the form of specimen's villi. Low, bounch or convoluted formed villi or gastric mucosal pattern was recognized under the dissecting microscope in the circumference of duodenal ulcer or at erosive changes. The shape of villi over the lymph follicle was flat or fused.2. Iron. Color of the upper part of normal shaped villi was changed from original white to light blue by prussian blue staining on iron introduction. Histological examination confirmed up take of iron into the duodenal epitelium.3. Fat Color of normal shaped villi was changed from original light orange to dark one by Sudan 111staining on fat introduction. Histological examination revealed that the quantity of fatty particles in the epithelial cytoplasm had increased after introduction of fat. 4. Decrease of iron and fat absorption was recognized in the circumference of duodenal ulcer. Conclusion: Absorbed substances into the duodenal mucosa were observed from mucosal surface. Methylene blue absorbed was recognized by endoscope. Absorbed iron and fat were detected under the dissecting microscopic observation of biopsy specimens with staining. The quantity of methylene blue, iron or fat uptake was diminished in some duodenal lesions accompanied by deformities of villi.
