Method validation and assessment of selected pesticide residues (Diazinon, Fenarimol, Cyanazine, Isoprothiolane, Simetryn) in water samples from Kurose River, Hiroshima prefecture, Japan

Abstract

This study evaluated method validation and five pesticide residues Diazinon, Fenarimol, Cyanazine, Isoprothiolane and Simetryn in water samples from Kurose River, Hiroshima prefecture, Japan. Water samples were collected every month (March to June 2016) from seven sites in Kurose river. The samples were concentrated with solid-phase extraction (SPE) apparatus and analyzed by a reverse phase HPLC. Assay validation parameters evaluated include linearity range, specificity, limit of detection (LOD) and limit of quantification (LOQ). Mobile phase compositions (Acetonitrile-MilliQ) were evaluated to optimize peak signal and retention times (RTs). Good linearity was achieved in a range of 10 to 200 ?g/L (r² ?0.99), with an accuracy range of 99.81 to 100.69%. The average recoveries of spiked samples ranged from 70.5 to 120.6%. The Cynazine and Simetryn registered relatively higher concentration during transplantation of rice (April and May), and correlated well with their application period. Sites K3 and K4 showed relatively higher concentrations during the month of June for almost all the pesticides, hence a need to evaluate their environmental risks.