A semi-dominant mutation in the gene encoding histidine kinase influences rice morphology

Abstract

Cytokinin plays a major role in the regulation of plant development. It is perceived by receptors with histidine kinase activity to regulate the expression of various transcription factors. In a previous study, we reported a semi-dominant mutant, named adaxial-abaxial bipolar leaf1 (abl1)-d, which exhibited a characteristic feature in the fourth leaf of rice, and that the ABL1 gene encodes a cytokinin receptor with histidine kinase activity. Our further analysis suggested that the abl1-d mutation is associated with an active form of histidine kinase and altered cytokinin signaling. However, it remained unclear whether the abl1-d mutation indeed triggers aberrant cytokinin signaling in rice plants, and how the abl1-d mutation affects developmental processes throughout the life cycle of rice. In the present study, we found that homozygous abl1-1d calli have the capacity to regenerate shoots in the absence of cytokinin, suggesting that the abl1-1d homozygous mutation is associated with constitutive cytokinin signaling in rice. We next examined morphological characteristics of both homozygous and heterozygous abl1-1d plants from the post-germination vegetative phase through to reproduction. The results showed that homozygous abl1-1d plants had a reduced number of panicles and were completely sterile, and that leaf size and the midrib structure were altered. Furthermore, the adaxial–abaxial bipolar leaf, a phenotype that is characteristic of the abl1-1d mutant, has previously been observed to resemble two normal leaves fused together at their abaxial sides. Leaves with this particular phenotype exhibited enhanced photosynthetic efficiency under certain environmental conditions. Thus, the abl1-1d mutation, which results in a putative active form of receptor histidine kinase, affects various developmental traits throughout the rice life cycle, probably due to altered cytokinin signaling.