Genes & Genetic Systems
Online ISSN : 1880-5779
Print ISSN : 1341-7568
ISSN-L : 1341-7568
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Detection of the Sec-1 locus of rye by a PCR-based method
Yuichiro ShimizuShuhei NasudaTakashi Ryu Endo
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1997 年 72 巻 4 号 p. 197-203

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The structural genes for the ω-secalins of rye (Secale cereale) are located in the Sec-1 locus on the short arm of rye chromosome 1R. We applied PCR (polymerase chain reaction) to detect the Sec-1 locus in a wheat genomic background. A primer set we designed based on a published sequence of a ω-secalin gene amplified not only the ω-secalin sequence, but also a putative ω-gliadin sequence. We determined partial sequences of both PCR-amplified fragments and designed different primers for the specific amplification of the ω-secalin sequence. One of the new primer sets amplified DNA fragments only in rye and wheat lines carrying chromosome 1R or telosome 1RS; no amplification occurred in either euploid wheats or 1RS deletion lines. This PCR-based method would provide efficient screening for the Sec-1 locus in progeny of wheat lines carrying chromosome 1R.

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© 1997 by The Genetics Society of Japan
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