Genes & Genetic Systems
Online ISSN : 1880-5779
Print ISSN : 1341-7568
ISSN-L : 1341-7568
Special reviews
New horizons in genome engineering of Drosophila melanogaster
Shu Kondo
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2014 Volume 89 Issue 1 Pages 3-8


Drosophila melanogaster has the longest history as a genetic model system and even in the present day remains the front runner in diverse fields of biology. However, lack of a convenient method to make specified modifications to endogenous genes has been a pain in the neck for many fly geneticists for decades. Synthetic nuclease technologies, especially the CRISPR/Cas9 system, hold great promise for a breakthrough. Synthetic nucleases are programmable nucleases that can be directed to cleave a specified sequence in the genome. Deleterious mutations can be efficiently induced by expression of a synthetic nuclease that targets a gene of interest. Precise modification of the target site, such as a reporter gene knock-in, is also possible by simultaneous delivery of a synthetic nuclease and a targeting vector. Here I summarize recent advances in synthetic nuclease technologies and discuss their possible applications to Drosophila genetics.

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© 2014 by The Genetics Society of Japan
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