MTK 肉腫 II, III およびVに及ぼす para- oxybenzoicacid ethylester の細胞学的影響
1960 年 35 巻 3 号 p. 89-94
詳細
1960 年 35 巻 3 号 p. 89-94
The present article deals with some cytological effects of para-oxybenzoicacidethylester on tumor cells of the MTK-sarcoma II, III and V, transplantable ascites tumors of rats, with particular regard to the morphological response of tumor cells to the drug. Intraperitoneal injections of the drug at dose level of 200, 300 and 400mg/kg were made in each tumor-bearing rat on the 3rd day after transfer of the tumor.
The drug seems to exert some injurious effects on nervous system of the treated animals, since they showed some difficulty in moving their hind legs for 10 to 30 minutes long after injection. The infiltration of tumor cells was observed in intraphragma and serosa of treated tumor animals, with hypertrophy in liver, spleen and kidney, though less remarkable in the latter two organs. The drug exerted a striking destructive influence on the tumor cells: 6-8 hours after injection, tumor cells were completely damaged in most cases studied. The effective duration of the drug seems to be comparatively short. Some tumor cells remained alive without damage by the drug: active tumor cells made their appearance 12 hours after injection, and formed a source of renewed tumor growth. Their life span was prolonged 1 to 2 days more.
Remarkable cytological effects of the drug on tumor cells are as follows: the deformation of nuclei and their resulting destruction were common in tumor cells after treatment. The reduction in stainability of nucleoli was remarkable in resting cells 1-2 hours after application. The drug seems to disintegrate nucleoli into tiny particles, resulting in the disturbance in function of the nucleus. Induced abnormalities of chromosomes leading to their disintegration are striking stickiness and aggregation at prophase and metaphase, and sticky bridges and lagging at ana-, telophase. The damage of the cytoplasm was also evident by the cytoplasmic swelling and blebbing of cell surface. In a more advanced condition the drug induced a formation of vacuoles in the cytoplasm.
