Abstract
1) Puffing patterns in the salivary gland chromosomes of Drosophila ananassae have been investigated.
2) The salivary chromosome map of this species was reconstructed.
3) Puffs were found 48 regions of a total of 6 elements of the salivary chromosomes during the 3rd instar and prepupal stages. Among these, 16 regions of the 2-chromosome were selected to observe changes in the puffing pattern through the developmental stages of this organism.
4) Comparison of the puffing patterns was made among three different karyotypes, AA, BB, and AB, derived from a subterminal inversion, In2L.
5) The appearance of puffs was found to be correlated with the rates of development of the three types, which varied in the relation AB=BB>AA.
6) No puff that was unique to any of the three karyotypes has been observed. Rather, in almost all regions the puff patterns of the three types were found to be similar with respect to both their correlation with the stages of development and their continuance.