A case of cytoplasmic male sterility controlled by a restoration gene
Rf was found in cultivated rice,
Oryza sativa L. The sterile cytoplasm [
ms] and the restoration gene
Rf were derived from Chinsurah Boro II, an
Indica variety, and the experiments were made by the isogenic lines having genetic background of Taichung 65, a
Japonica variety.
When a plant with sterile cytoplasm [
ms] had
Rf Rf, it was completely male-fertile; when it had
Rf rf, partially male-fertile (ca. 50%); and when it had
rf rf, completely male-sterile. Plants with normal cytoplasm [
ms+] would be male-fertile regardless of the genotype for restoration genes. The F
1 plants of [
ms] rf rf×[ms+]
Rf rf segregated into partially male-sterile and completely male-sterile classes in a 1:1 ratio, while the selfed progeny of [
ms+] Rf rf was completely male-fertile. The restoration gene was thus found to be of gametophytic type.
The F
1 plants of [
ms] rf rf×Rf Rf had a 90% or higher seed set, though they were partially male-sterile. This source may be used for breeding “hybrid rice”.
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