抄録
We present evidence demonstrating the importance of tissue uric acid determination by liquid chromatography. Uric acid in mammalian tissues was determined by reversed-phase high-performance liquid chromatography (RPHPLC) with electrochemical detection (ECD) recently developed by us. The chromatographic examinations revealed marked over-production of uric acid in human malignant tumor tissues and also regional over-production in the left hemisphere following left middle cerebral artery occlusion. In some diseases, hyperuricemia may result in marked over-production of uric acid in tissues. Therefore, uric acid levels in body fluids were investigated in relation to diseases. The HPLC-ECD method allows determination of uric acid levels in body fluids as well as in mammalian tissues up to 10 pg. We are now investigating the relationship between abnormalities in purine metabolism in mammalian tissues and diseases.
